US Patent:
20070275481, Nov 29, 2007
Inventors:
Elena Vasilyeva - Swampscott MA, US
Francine Brown - Amherst NH, US
Matthias Kretschmer - Weston MA, US
Peter Bove - Burlington VT, US
Hans Fajardo - Wakefield MA, US
Frederick Taylor - Milton MA, US
Rohin Mhatre - Lexington MA, US
Kazumi Kobayashi - Arlington MA, US
Amy Dingley - Roslindale MA, US
Assignee:
BIOGEN IDEC MA INC. - CAMBRIDGE MA
International Classification:
G01N 33/561
C07K 1/00
C07K 14/00
C07K 16/18
G01N 33/00
US Classification:
436516000, 436086000, 530350000, 530387100, 530409000
Abstract:
The invention provides a method of using chip-based gel electrophoresis to determine the presence of polypeptides with selected disulfide linkage patterns, for example, completely formed tetrameric antibodies as compared to incompletely formed heterodimeric half-antibodies. The invention further features a kit, comprising a chip, and instructions for conducting the foregoing method. The methods and kits of the invention are amendable to high throughput applications for the monitoring of production and quality control of recombinant therapeutic antibodies.