US Patent:
20110110888, May 12, 2011
Inventors:
Hai-Qing Xian - Foster City CA, US
Jian Q. Yao - Austin TX, US
Hali Wang - The Hills TX, US
Massoud Daheshia - Cedar Park TX, US
Hui Liu - Austin TX, US
International Classification:
A61K 38/20
C12N 5/071
C12M 3/00
A61K 38/18
A61K 38/22
A61K 38/39
A61K 38/29
A61K 38/28
A61K 38/26
A61K 38/23
A61K 38/19
A61K 38/21
A61P 43/00
US Classification:
424 852, 435395, 4352891, 514 76, 514 97, 514 88, 514 89, 514 91, 514 82, 514 85, 514 96, 514 81, 514 172, 514 93, 514 2192, 514 118, 514 59, 514 117, 514 119, 424 851, 424 854, 514 77, 514 78
Abstract:
A system and method for producing a scaffold coated and/or impregnated with at least one bioactive agent. This is accomplished by culturing at least one cell in the same medium as a scaffold, but without physical contact between the at least one cell and the scaffold. The system includes a container having a surface defining an interior compartment. A medium is disposed within the interior compartment. At least one cell is disposed within the medium, the at least one cell being capable of producing at least one bioactive agent. Further, a scaffold is disposed within the medium. The scaffold is physically separated from the at least one cell such that there is no contact between the scaffold and the at least one cell. Thus, as the at least one cell produces at least one bioactive agent, such as a growth factor, the at least one bioactive agent enters the medium, and contacts and coats and/or impregnates the scaffold. However, due to the physical separation of the scaffold and at least one cell, the at least one cell is prevented from contacting and coating and/or impregnating the scaffold. Thus, only the desired bioactive agent or agents coat and/or impregnate the scaffold.