US Patent:
20120064117, Mar 15, 2012
Inventors:
Ted M. Ross - Pittsburgh PA, US
Xianchun Tang - West Roxbury MA, US
Hairong Lu - West Roxbury MA, US
International Classification:
A61K 39/145
C12N 7/01
C12N 15/866
C12N 5/10
A61P 31/16
A61P 37/04
US Classification:
4242101, 4353201, 435348, 4352351
Abstract:
Disclosed herein is the finding that baculovirus display of multiple influenza virus hemagglutinin (HA) proteins elicits broadly reactive immune responses against influenza. Thus provided herein are recombinant baculovirus vectors having a first, second, third and fourth nucleic acid sequence, each encoding an influenza hemagglutinin (HA) fusion protein. The first, second, third and fourth nucleic acid sequences each encode an influenza HA with a different amino acid sequence. Also provided are recombinant baculoviruses displaying a first, second, third and fourth influenza virus HA fusion protein in the baculovirus envelope, wherein each HA fusion protein comprises a different HA amino acid sequence. Tetravalent influenza virus vaccines comprising the recombinant baculoviruses disclosed herein are further provided. In addition, methods of immunizing a subject against influenza virus using the tetravalent influenza virus vaccines are provided. In particular examples of the compositions and methods disclosed herein, the HA polypeptides are from H5N1 influenza virus.