Gloria E Sarto

57503400, May 12, 1998

Apr 7, 1995

8/418704

In C. Kim - Albuquerque NM
Donald M. Thompson - Albuquerque NM
Gloria E. Sarto - Albuquerque NM

University of New Mexico - Alburquerque NM

C12Q 168
A61K 944
G01N 130

435 6

A fluorescence in situ hybridization (FISH) procedure and solutions is provided. The entire FISH procedure is fast (15 minutes or less), with the hybridization step occurring in 5 minutes or less. The entire FISH procedure with digoxygenin- or biotin-labeled probes takes approximately 30 minutes. A formamide-free solution (dextran sulfate and glycerol) is provided. An additional solution (10% dextran sulfate and 20% formamide) is also provided. Additional solutions are provided for mRNA in situ hybridization whereby the process takes less than 24 hours.

60226897, Feb 8, 2000

Oct 9, 1996

8/727951

Gloria E. Sarto - Albuquerque NM
Donald M. Thompson - Albuquerque NM

University of New Mexico - Albuquerque NM

C12Q 168

435 6

A method of preparing flourescent in situ hybridization slides, comprising: providing a slide with a raised ring thereon; providing to the slide a DNA probe; providing to the slide cells and target DNA; providing to the slide a formamide-free mixture of 10%. +-. 2% by weight dextran sulfate and 15%-25% glycerol and 0. 9% by weight salt or a solution of 10%. +-. 2% by weight dextran sulfate, 10-30% by volume formamide and 0. 9% by weight salt; placing a coverglass on the ring; denaturing and hybridizing the slide; and performing a post-hybridization wash of the slide.