Inventors:
Gerard Gundling - Lake Forest IL, US
Thomas Laffler - Vista CA, US
Cristina A. Ivy - San Jose CA, US
Lendell Cummins - San Diego CA, US
Assignee:
IBIS BIOSCIENCES, INC. - Carlsbad CA
International Classification:
C12N 15/10
US Classification:
435209, 435270, 435220, 4353061
Abstract:
The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.