Gary L Chrebet

58213538, Oct 13, 1998

Aug 1, 1996

8/619554

Cameron M. Douglas - Piscataway NJ
Gary L. Chrebet - Princeton NJ
Joseph Clemas - Metuchen NJ
Mohammed El-Sherbeini - Westfield NJ
Forrest Foor - New York NY
Jennifer Nielsen Kahn - East Brunswick NJ
Rosemarie Kelly - Westfield NJ
Jean A. Marrinan - Somerset NJ
Nancy R. Morin - Cranford NJ
Janet C. Onishi - Westfield NJ
Stephen Authur Parent - Cranbury NJ
Naasa M. Ramadan - Westfield NJ
Elizabeth A. Register - Scotch Plains NJ

Merck & Co., Inc. - Rahway NJ

C07H 2104
C12N 115
C12P 2102
C07K 14395

536 2374

DNA molecules encoding proteins involved in biosynthesis of 1,3-beta-D glucan are identified, cloned, expressed and used in in vitro assays to screen for antifungal compounds, including compounds that affect cell wall biosynthesis. The invention includes but is not limited to the purified DNA molecules, assays employing the DNA molecules, proteins encoded by the DNA molecules, cells expressing the DNA molecules and altered forms of the molecules.

55873227, Dec 24, 1996

Nov 23, 1994

8/344217

Gary L. Chrebet - Princeton NJ

ReplicaTech, Inc. - Princeton NJ

C12M 126
C12M 132

4353094

A device for replica plating of cellular colonies. The device has a solid body with a first region and a first end coupled to a peripheral ridge. A ridge is disposed at the transition from the first region to the peripheral ridge. A sheet of material capable of transferring cellular colonies from one medium to another is placed over the first end and the first region. A locking element defining an aperture passes over the first region and the sheet of material creating a frictional fit which pulls the sheet of material taut over the first end. The sheet of material is compressed between the locking element and the peripheral ridge thereby holding the sheet of material in place.

53246594, Jun 28, 1994

Jul 31, 1992

7/923082

Stephen A. Parent - Cranbury NJ
Leonardo E. Brizuela - Heidelberg, DE
Gary L. Chrebet - Princeton NJ
Keith A. Bostian - Westfield NJ

Merck & Co., Inc. - Rahway NJ

C12N 116

4352552

Disclosed is a process utilizing yeast mutants for identifying active FK-506 type immunosuppressants. The process utilizes Saccharomyces cerevisiae mutants containing an fkr1, fkr2, fkr3 mutant gene or mixture thereof, which are resistant to FK-506 but sensitive to rapamycin. These mutants can be used in a diagnostic procedure for identifying FK-506 and FK-506 type immunosuppressants and in screening assays for compounds and fermentation broths which exhibit FK-506 type immunosuppressive activity. Specifically disclosed are the new yeast mutants, Saccharomyces cerevisiae YKF093, (Merck Culture Collection No. MY 2088) ATCC No. 74055, containing an fkr3 mutant gene, Saccharomyces cerevisiae YKF 012, (Merck Culture Collection No. MY 2096) ATCC No. 74061, which contains an fkr1 mutant gene, Saccharomyces cerevisiae YFK 014, (Merck Culture Collection No.