US Patent:
20210214388, Jul 15, 2021
Inventors:
- RIXENSART, BE
Yingxia WEN - Cambridge MA, US
Andrew GEALL - Cambridge MA, US
Frederick PORTER - Holly Springs NC, US
Assignee:
GLAXOSMITHKLINE BIOLOGICALS SA - RIXENSART
International Classification:
C07H 21/02
B01D 15/36
B01D 15/38
C07H 1/06
C12N 15/10
B01D 61/14
Abstract:
Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.
