Flordeliza S Villanueva

20020022266, Feb 21, 2002

Jun 28, 2001

09/894547

William Wagner - Wexford PA, US
Flordeliza Villanueva - Pittsburgh PA, US
Timothy Deglau - Monroeville PA, US

C12N005/00

435/325000

Methods for delivering drugs, vectors, cells diagnostic agents and other chemical or biological entities to a tissue or cellular surface are disclosed. The methods generally involve coupling a molecule to the tissue or cellular surface. The molecule comprises at least one reactive group that reacts with groups present on the surface, and at least one signaling molecule. A chemical or biological entity having a recognition molecule attached thereto or expressed thereby is introduced to the surface. The recognition molecule and signaling molecule bind so as to attach the entity to the surface. Localized delivery of various chemical and biological entities is thereby achieved. The molecule can also contain a polymer that can mask adhesive information inherent to the tissue or cellular surface. Tissues and cells modified according to the present methods are also disclosed.

20100158815, Jun 24, 2010

Nov 5, 2009

12/613400

Jianjun Wang - Glenshaw PA, US
Flordeliza Villanueva - Pittsburgh PA, US
Xucai Chen - Glenshaw PA, US
Andrew Fisher - Pittsburgh PA, US
William Richard Wagner - Wexford PA, US

A61B 8/08

424 952

This invention addresses the clinical problem of how to optimize biological cell based therapies, such as stem cell therapy. Currently, cell therapies administered by intravenous, intra-arterial, and/or direct tissue injection are limited by the lack of clinically available imaging methods to detect the in vivo fate of the administered cells. There are many efforts underway to develop imaging strategies for stem cells in vivo, including radionuclide and MRI-based approaches. However, these approaches are limited by potential safety issues (e.g. radioactive exposure of stem cells, toxicity of iron particles used for MRI) and difficulty in serial tracking due to complex instrumentation and/or the requirement for repetitive radiation exposure.

20110208113, Aug 25, 2011

Sep 13, 2010

12/880807

Catalin Toma - Pittsburgh PA, US
Flordeliza Villanueva - Pittsburgh PA, US
William Richard Wagner - Wexford PA, US
Joon S. Lee - Pittsburgh PA, US
Jianjun Wang - Glenshaw PA, US
Xucai Chen - Glenshaw PA, US
Andrew Fisher - Pittsburgh PA, US

A61K 35/12
A61M 37/00
C12N 11/00

604 22, 435174, 424 937

The disclosed technology describes compositions and methods useful for providing cell based therapy. For example, one embodiment of cell based therapy involves the regeneration of injured tissue and/or promoting wound healing. Certain embodiments provide improved therapeutic compositions using microbubbles by delivering biological progenitor cells to the injured tissues. The administration of the microbubbles is directed by acoustic radiation forces that interact with embodiments of microbubbles comprising an acoustically active gas. As such, a high efficiency of progenitor cell delivery to injured tissue is realized. One advantage of this technique over targeted delivery of pharmaceutical compounds, is that the delivered progenitors cells may be derived from the patient (i.e., personalized therapy), thereby avoiding side effects, allergic reactions, and overall problems associated with refractive drug responses.

20230065014, Mar 2, 2023

Jan 14, 2021

17/792653

- Pittsburgh PA, US
Bruce A. Freeman - Pittsburgh PA, US
Flordeliza S. Villanueva - Pittsburgh PA, US
John J. Pacella - Bethel Park PA, US
Marco Fazzari - Pittsburgh PA, US
Xucai Chen - Pittsburgh PA, US

A61K 9/50
A61K 47/10
A61K 47/24
A61K 31/19
A61P 9/10

Provided herein are microbubble compositions comprising nitro-fatty acids and/or esters thereof, such as amphiphilic esters or allyl esters thereof. Also provided are methods of reducing local inflammation at a site in a patient comprising delivering the microbubbles to a site of inflammation in the patient and applying ultrasound to the microbubbles. The methods may be used to treat fibrosis or cancer.

20170156699, Jun 8, 2017

Feb 14, 2017

15/432873

- Pittsburgh PA, US
Francois T.H. Yu - Pittsburgh PA, US
Flordeliza S. Villanueva - Pittsburgh PA, US

University of Pittsburgh - Of the Commonwealth System of Higher Education - Pittsburgh PA

A61B 8/08
G01S 7/52
A61M 5/00
A61K 49/22
A61B 8/12
A61B 8/00

A method of imaging a blood vessel includes delivering a bubble-based contrast agent within the vessel and positioning at least one ultrasound device in the vicinity of the bubble-based contrast agent within the vessel. A first burst of low-frequency ultrasound energy can be delivered to excite the bubble-based contrast agent into oscillation within the vessel, and a second burst of high-frequency ultrasound energy can be delivered at the excited bubble-based contrast agent. A return signal from the burst of high-frequency ultrasound energy can be received and processed to obtain one or more images.

20150141817, May 21, 2015

Jun 27, 2013

14/409409

- Pittsburgh PA, US
Jianjun Wang - Pittsburgh PA, US
John J. Pacella - Pittsburgh PA, US
Flordeliza S. Villanueva - Pittsburgh PA, US

A61B 8/08
A61M 5/00
A61K 49/22

600431

Various methods of performing ultrasound contrast assisted therapy are provided. One such method includes delivering a plurality of microbubble-based ultrasound contrast agents to a target area and disrupting the microbubble-based ultrasound contrast agents by delivering tone bursts of ultrasound to the target area. The oscillation of the microbubble-based ultrasound contrast agents can be achieved by delivering ultrasound tone bursts of greater than 5 acoustic cycles with a pulse repetition frequency of between 0.01 and 20 Hz, with pressure greater than 0.3 MPa.

20140236005, Aug 21, 2014

Aug 24, 2012

14/241051

Xucai Chen - Pittsburgh PA, US
Francois T.H. Yu - Pittsburgh PA, US
Flordeliza S. Villanueva - Pittsburgh PA, US

University of Pittsburgh-Of the Commonwealth Systems of Higher Education - Pittsburgh PA

A61B 8/08
A61B 8/00
A61B 8/12

600433, 600431

A method of imaging a blood vessel includes delivering a bubble-based contrast agent within the vessel and positioning at least one ultrasound device in the vicinity of the bubble-based contrast agent within the vessel. A first burst of low-frequency ultrasound energy can be delivered to excite the bubble-based contrast agent into oscillation within the vessel, and a second burst of high-frequency ultrasound energy can be delivered at the excited bubble-based contrast agent. A return signal from the burst of high-frequency ultrasound energy can be received and processed to obtain one or more images.