US Patent:
20170267970, Sep 21, 2017
Inventors:
- Cambridge MA, US
Daniel MILLER - Somerville MA, US
Ethan SOKOL - Somerville MA, US
Assignee:
Whitehead Institute for Biomedical Research - Cambridge MA
International Classification:
C12N 5/00
A61L 27/26
A61L 27/54
A01K 67/027
C12N 5/071
G01N 33/50
C12N 5/09
C12N 5/0793
A61L 27/52
A61L 27/38
Abstract:
The presently disclosed subject matter provides hydrogel precursor compositions (e.g., solutions) for forming three-dimensional hydrogels that support growth of physiologically relevant tissue when at least one cell is cultured in the three-dimensional hydrogel, kits comprising the hydrogel precursor composition, three-dimensional hydrogels, methods of forming the three-dimensional hydrogels, methods of growing the physiologically relevant tissue using the three-dimensional hydrogels, physiologically relevant tissue grown in the three-dimensional hydrogels, methods of producing hormone-responsive tissue (e.g., milk-producing mammary tissue and related methods of producing milk), methods of screening for candidate agents useful for modulating hormonal responses (e.g., modulating milk production), method of screening for candidate therapeutic agents using the physiologically relevant tissue grown in the three-dimensional hydrogels (e.g., personalized cancer treatments), and related methods of treatment (e.g., administering agents identified using the methods herein, transplanting physiologically relevant tissue produced using the methods, etc.).