Resumes
Resumes
Enju Wang
View pageLocation:
United States
Enju Wang Phones & Addresses
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22043 77Th Ave, Oakland Gdns, NY 11364
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7510 173Rd St, Fresh Meadows, NY 11366 (718) 969-6335 (607) 351-6631 (718) 969-7341
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Bayside, NY
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Ann Arbor, MI
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Queens, NY
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Oakland Gdns, NY
Publications
Us Patents
Optical Membrane Films For Polycation Detection
View pageUS Patent:
61562742, Dec 5, 2000
Filed:
Feb 26, 1998
Appl. No.:
9/032503
Inventors:
Enju Wang - Flushing NY
Assignee:
St. John's University - Jamaica NY
International Classification:
G01N 2141
G01N 2101
G01N 3116
G01N 2100
C07D31182
G01N 2101
G01N 3116
G01N 2100
C07D31182
US Classification:
422 8206
Abstract:
Lipophilic fluorescein-based molecules have been synthesized and incorporated into thin plasticized polymeric membrane films as chromoionophores for the optical sensing and detection of polycationic protamine. The membrane response is based on the extraction of protamine into the film due to the interaction between the anionic fluorescein and the protamine polycation which results in a cation exchange between the protamine and proton, and thus, induces an absorbance spectra change of the polymeric film. The response speed is controlled by protamine diffusion through the stagnant diffusion layer adjacent to the film surface as well as within the bulk of the polymer film. When limited exposure time and non-stirring detection modes are used in a buffer solution, absorbance of a film changes as a function of the protamine concentration in the range of about 2 to 60. mu. g/ml (0. 44 to 13. 3. mu. M). The sensing film shows good selectivity over most common small cations, it can be used in the determination of protamine in diluted serum or whole blood. No response is observed when a protamine complexing reagent such as heparin is present, thus the instant invention is useful as an indicator for the protamine-heparin titration.