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Duane L Inlow

from Vista, CA
Age ~73
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Also known as:
Duane Leland Inlow, Duane Jennifer Inlow, Duane L Inco, Duane W
Phone and address:
172 Calle Huerto, Vista, CA 92084
(442) 224-7431
Related to:
Carmen A Alarcon, 44Lucila Rubalcava, 63Cheryl Travagline, 36Linda Grossman
Connected to:
Andrew J Inlow, 47Edwin Inlow, 72Olivia K Inlow, 32Shaun M Inlow, 35Alicia M Inman, 51Alissa C Inman, 43Alix H Inman, 36Angela L Inman, 49Antoine L Inman, 41April D Inman, 42

Duane Inlow Phones & Addresses

  • 172 Calle Huerto, Vista, CA 92084 (442) 224-7431
  • Southborough, MA
  • Boulder Creek, CA
  • 221 Smith Rd, Alamo, CA 94507
  • 12 Pacer Pl, Walnut Creek, CA 94596
  • Albany, CA
  • Oakland, CA
  • Santa Cruz, CA

Resumes

Resumes

Duane Inlow Photo 1

Duane Inlow

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Location:
221 Smith Rd, Alamo, CA 94507

Publications

Us Patents

Method Of Increasing Product Expression Through Solute Stress

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US Patent:
6905847, Jun 14, 2005
Filed:
May 30, 2001
Appl. No.:
09/867948
Inventors:
Brian Maiorella - Oakland CA, US
Duane Inlow - Oakland CA, US
William Howarth - Richmond CA, US
Assignee:
Chiron Corporation - Emeryville CA
International Classification:
C12P021/08
C12N005/16
US Classification:
435 7021, 435 691, 435 701, 4352401, 43524021, 4351721, 4353201, 435325, 4353871, 435440, 530350, 5303871, 536 231
Abstract:
A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One-to three-fold increases in antibody yield have been obtained by these methods.

Method Of Increasing Product Expression Through Solute Stress

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US Patent:
7070960, Jul 4, 2006
Filed:
May 9, 2005
Appl. No.:
11/124746
Inventors:
Brian Maiorella - Oakland CA, US
Duane Inlow - Oakland CA, US
William Howarth - Richmond CA, US
Assignee:
Chiron Corporation - Emeryville CA
International Classification:
C12P 21/08
C12P 21/00
US Classification:
435 7021, 435 691, 435 701, 4352401, 4351721, 435440, 530350, 5303871
Abstract:
A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.

Method Of Purifying Tfpi And Tfpi Analogs

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US Patent:
7851433, Dec 14, 2010
Filed:
Jan 8, 2004
Appl. No.:
10/753078
Inventors:
David H. Reifsnyder - El Cerrito CA, US
Duane Inlow - Alamo CA, US
Glenn Dorin - San Rafael CA, US
Patricio T. Riquelme - Walnut Creek CA, US
Cynthia A. Cowgill - Berkeley CA, US
Douglas G. Bolesch - Berkeley CA, US
Mark E. Gustafson - St. Charles MO, US
Assignee:
Novartis Vaccines and Diagnostics, Inc. - Emeryville CA
Pharmacia & Upjohn Company - Kalamazoo MI
International Classification:
C07K 14/00
US Classification:
514 2, 530350
Abstract:
Highly purified preparations of TFPI or TFPI analogs can be prepared using a method that generally involves the following steps: (1) expression of TFPI or TFPI analog in , (2) isolation of refractile bodies, (3) dissolution of the refractile bodies and refolding of the expressed TFPI or TFPI analog, (4) SP-Sepharose fast flow (FF) chromatography, (5) a first concentration and diafiltration step, (6) Q-Sepharose high (HP) performance chromatography, (7) butyl hydrophobic interaction chromatography (HIC), (8) SP-Sepharose HP chromatography, and (9) a second concentration/diafiltration step. Less than about 12% of the TFPI or TFPI analog molecules in such preparations are modified TFPI or TFPI analog species (i. e. , oxidized, carbamylated, acetylated, deamidated, aggregated, or misfolded species).

Cell Culture Medium For Enhanced Cell Growth, Culture Longevity, And Product Expression

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US Patent:
60487283, Apr 11, 2000
Filed:
Jun 7, 1995
Appl. No.:
8/480508
Inventors:
Duane Inlow - Oakland CA
Brian Maiorella - Oakland CA
William Howarth - Castro Valley CA
Assignee:
Chiron Corporation - Emeryville CA
International Classification:
C12N 500
US Classification:
435404
Abstract:
Protein-free cell culture media supplements are described consisting of synergistic combinations of medium components, which when added to cell culture media, either serum supplemented or serum-free, enhance cell growth, culture longevity and product expression.

Method Of Increasing Product Expression Through Solute Stress

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US Patent:
62388916, May 29, 2001
Filed:
Jun 7, 1995
Appl. No.:
8/482421
Inventors:
Brian Maiorella - Oakland CA
Duane Inlow - Oakland CA
William Howarth - Richmond CA
Assignee:
Cetus Oncology Corporation - Emeryville CA
International Classification:
C12P 2108
C12N 516
US Classification:
435 7021
Abstract:
A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.

Serum Free Media For The Growth On Insect Cells And Expression Of Products Thereby

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US Patent:
50249479, Jun 18, 1991
Filed:
Jul 24, 1987
Appl. No.:
7/077303
Inventors:
Duane Inlow - Oakland CA
Brian Maiorella - Oakland CA
Assignee:
Cetus Corporation - Emeryville CA
International Classification:
C12N 500
US Classification:
43524031
Abstract:
Serum-free media which support the growth of insect cells and the production thereby of recombinant proteins and viral products are herein disclosed. The serum free media can support insect cell growth at large scale under agitated and/or sparged, preferably well-aerated conditions. The serum free medium disclosed support insect cell growth to cell densities comparable to serum-containing culture and the production of viral and recombinant products to levels equivalent to those found in serum containing culture.

Methods For Adapting Cells For Increased Product Production Through Exposure To Ammonia

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US Patent:
51569647, Oct 20, 1992
Filed:
Jan 21, 1992
Appl. No.:
7/824694
Inventors:
Duane Inlow - Oakland CA
Brian Maiorella - Oakland CA
Andrea E. Shauger - Albany CA
Assignee:
Cetus Corporation - Emeryville CA
International Classification:
C12N 1500
C12N 500
C12N 502
C07K 300
US Classification:
4351721
Abstract:
This invention is in the field of cell and/or tissue culture. In particular, this invention relates to methods which adapt cells to a desired phenotype by exposing the cells to high levels of ammonia in culture, and subsequently transferring the adapted cells to a new culture medium in which there is no initial level of ammonia or the initial level of ammonia is below the level to which cells have been exposed to during the adaptation process. In this new culture medium, the adapted cells express the desired phenotype of growing to a higher viable cell density, and/or remaining viable for a longer period of time, and/or producing more of a desired cell product than their non-adapted counterparts grown in the same medium. This invention includes the adapted cells produced thereby and their cell products.

Lipid Microemulsions For Culture Media

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US Patent:
53729439, Dec 13, 1994
Filed:
Jul 12, 1993
Appl. No.:
8/090568
Inventors:
Duane Inlow - Oakland CA
Brian Maiorella - Oakland CA
Assignee:
Cetus Corporation - Emeryville CA
International Classification:
C12N 500
US Classification:
43524031
Abstract:
Lipid microemulsions which can be added to cell culture media to provide essential lipids in a bioavailable form and their components are disclosed. Methods to disperse lipids in culture media by the use of one or more emulsifiers are described. Further disclosed are media which support growth of cells and production of recombinant, viral and/or native products wherein lipids are supplied in the form of microemulsion.
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Duane L Inlow from Vista, CA, age ~73 Get Report