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Dring Crowell Phones & Addresses

  • 11991 Hollyhock Dr, Fishers, IN 46037
  • 12715 Charing Cross Rd, Carmel, IN 46033 (317) 815-1299
  • Pocatello, ID
  • Indianapolis, IN
  • Madison, WI
  • 13540 Shakamac Dr, Carmel, IN 46032

Work

Position: Production Occupations

Education

Degree: Associate degree or higher

Publications

Us Patents

Expression Library Screen By Prenylation Of Expressed Proteins

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US Patent:
59324325, Aug 3, 1999
Filed:
Jan 21, 1997
Appl. No.:
8/785795
Inventors:
Dring N. Crowell - Indianapolis IN
Brenda Biermann - Zionsville IN
Stephen Randall - Speedway IN
Assignee:
Indiana University Foundation - Bloomington IN
International Classification:
C12Q 148
C12Q 168
G01N 3358
US Classification:
435 15
Abstract:
Described is a novel in vitro method for obtaining and identifying proteins which, in their natural in vivo setting, are covalently modified after translation. To identify novel isoprenylated proteins for subsequent biochemical study, colony blots of a Glycine max cDNA expression library were �. sup. 3 H! farnesyl labeled in vitro. Proteins identified by this screen contained several different carboxy-termini that conform to consensus farnesylation motifs. These proteins included known farnesylated proteins (DnaJ homologs) and several novel proteins, two of which contained 6 or more tandem repeats of a hexapeptide having the consensus sequence �E or G! �G or P!EK�P or K!K. Expression library screening by direct labeling can thus be adapted to recover and identify isoprenylated proteins as well as proteins with other post-translational modifications. This identification and recovery further enables the recovery of transformants containing DNA encoding the proteins, as well as the raising of antibodies to the proteins.
Dring N Crowell from Fishers, IN, age ~67 Get Report