Demian Forest Obregon

20130217045, Aug 22, 2013

Feb 15, 2013

13/768158

Jun Tan - Tampa FL, US
Demian Obregon - Tampa FL, US
Huayan Hou - Tampa FL, US
Juan Deng - Tampa FL, US

University of South Florida (A Florida Non-Profit Corporation) - Tampa FL

G01N 33/68

435 792, 435 71

Provided herein is a method for diagnosing Alzheimer's disease in a subject comprising detecting an increase in an amyloidogenic Aβantibody in the subject as compared to a control. Further provided herein is a method for testing efficacy of an Alzheimer's disease treatment in a subject comprising detecting a decrease in an amyloidogenic Aβantibody in the subject as compared to prior to the treatment.

20130291135, Oct 31, 2013

Jun 25, 2013

13/926610

Jun Tan - Tampa FL, US
Demian Forest Obregon - Tampa FL, US
Huayan Hou - Tampa FL, US

UNIVERSITY OF SOUTH FLORIDA - Tampa FL

A01K 67/027

800 12, 800 22

Evidence indicates dysregulation. of the immunoregulatory molecule CD45 occurs in Alzheimer's disease (AD). Transgenic mice overproducing amyloid-β peptide (Aβ) and deficient in CD45 (PSAPP/CD45/) recapitulate AD neuropathology. Increased cerebral intracellular and extracellular soluble oligomeric and insoluble Aβ, decreased plasma soluble Aβ increased microglial neurotoxic cytokines TNF-α and IL-1β, and neuronal loss were found in PSAPP/CD45/ mice compared with CD45-sufficient PSAPP littermates. After CD45 ablation, in vitro and in vivo studies demonstrate a microglial phenotype whereby microglia phagocytose less Aβ but display proinflammatory properties. This microglial activation occurs with elevated Aβ oligomers and neural injury and loss as determined by decreased ratio of anti-apoptotic Bcl-xL to proapoptotic Bax, increased activated caspase-3, mitochondrial dysfunction, and loss of cortical neurons in PSAPP/CD45/ mice. These data show that deficiency in CD45 activity leads to brain accumulation of neurotoxic Aβ oligomers and validate CD45-mediated microglial clearance of oligomeric Aβ as a novel AD therapeutic target.

20130165339, Jun 27, 2013

Jun 30, 2011

13/704921

Jun Tan - Tampa FL, US
Demian Obregon - Tampa FL, US
Antoinette Bailey - Tampa FL, US

UNIVERSITY OF SOUTH FLORIDA - TAMPA FL

G01N 33/68

506 9, 436501, 435 792, 435 79

The subject invention pertains to the use of amyloid precursor protein-alpha (sAPP-α) as a biomarker for prediction of a subject's risk of developing inflammatory and/or autoimmune-related disorders. In addition, the present invention provides methods for optimizing vaccine schedules and compositions, thereby preventing or reducing the risks of vaccine-induced inflammatory and/or autoimmune-related disorders.

20150153330, Jun 4, 2015

Nov 24, 2014

14/551958

JUN TAN - TAMPA FL, US
DEMIAN OBREGON - TAMPA FL, US
ANTOINETTE BAILEY - PASADENA CA, US

UNIVERSITY OF SOUTH FLORIDA - TAMPA FL

G01N 33/50

The subject invention pertains to the use of amyloid precursor protein-alpha (sAPP-α) as a biomarker for prediction of a subject's risk of developing inflammatory and/or autoimmune-related disorders. In addition, the present invention provides methods for optimizing vaccine schedules and compositions, thereby preventing or reducing the risks of vaccine-induced inflammatory and/or autoimmune-related disorders.

20070110722, May 17, 2007

Dec 12, 2005

11/164963

Demian Obregon - St. Petersburg FL, US
Mussa Ali - St. Petersburg FL, US

A61K 48/00
C12Q 1/68
C12P 19/34
C12N 15/86

424093200, 435006000, 435091200, 435455000, 435456000, 977802000

Methods for the production of duplexes and single-stranded RNA and/or DNA of a desired length and sequence based on a novel template design which incorporates 2 polymerase promoters, primers, and production sequences within a single molecule are provided. This Single-stranded Template molecule design allows high-efficiency, high-yield production of single or multiple nucleic acid molecules in a single reaction vessel and thus is amenable to high-throughput automation. This Single-stranded Template molecule design also allows easy incorporation of Single-stranded Template molecules into delivery vectors for either in vitro, ex vivo, in vivo, or therapeutic application. Methods for producing Single stranded Template molecule-based RNA or DNA molecules, or hybrid molecules, in vivo and therapeutic uses for such molecules are provided. Single-stranded Template molecule kit designs are also described.

20060205078, Sep 14, 2006

Dec 14, 2005

11/306044

Demian Obregon - St. Petersburg FL, US
Mussa Ali - St. Petersburg FL, US

C12N 15/74
C12N 15/63
C12N 15/82

435455000, 435471000, 435468000

A method is provided for the induction of aberrant RNA interference (abRNAi) by introducing ‘aberrant’ RNAs into the cell wherein a long-stranded antisense or sense RNA is simultaneously introduced with a short 4-12 mer homologous or complementary or random RNA wherein the long-short RNAs induce sequence specific inhibition of the homologous gene within a cell. The invention disclosed herein can be used to suppress gene expression in vitro, ex vivo or in vivo.

20060199191, Sep 7, 2006

Dec 12, 2005

11/164961

Demian Obregon - St. Petersburg FL, US
Mussa Ali - St. Petersburg FL, US

C12Q 1/68
C12P 19/34

435006000, 435091200

Methods for the production of duplexes and single-stranded RNA and/or DNA of a desired length and sequence based on a novel template design which incorporates 2 polymerase promoters, primers, and production sequences within a single molecule are provided. This single molecule template design allows high-efficiency, high-yield production of single or multiple nucleic acid molecules in a single reaction vessel and thus is amenable to high-throughput automation. This single molecule design also allows easy incorporation of single molecule templates into delivery vectors for either in vitro, ex vivo, in vivo, or therapeutic application. Methods for producing single template molecule-based RNA or DNA molecules, or hybrid molecules, in vivo and therapeutic uses for such molecules are provided. Single molecule template kit designs are also described.