US Patent:
20120220751, Aug 30, 2012
Inventors:
Gregory J. Ewing - , US
Deborah Kay Fieri - Oceanside CA, US
Khairuzzaman Bashar Mullah - Union City CA, US
Ronald J. Graham - , US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C07K 2/00
C07C 291/08
Abstract:
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET.Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.