Deborah B Boles

8606529, Dec 10, 2013

Mar 26, 2012

13/429911

Deborah Boles - Cary NC, US
Kenton C. Hasson - Germantown MD, US
Sami Kanderian - Germantown MD, US

Canon U.S. Life Sciences, Inc. - Rockville MD

G06F 19/00

702 19, 435 6, 435 71, 435 79, 435 911, 435 912, 544245, 544334, 544242, 544152, 536 231, 536 243

The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

20130178374, Jul 11, 2013

Jul 6, 2012

13/542721

Allen E. Eckhardt - Durham NC, US
Jonathan Benton - Apex NC, US
Deborah Boles - Cary NC, US

ADVANCED LIQUID LOGIC, INC. - Research Triangle Park NC

C12N 15/10
C12Q 1/68

506 5, 506 38

The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

20180321170, Nov 8, 2018

May 25, 2018

15/990369

- Rockville MD, US
Deborah John Boles - Cary NC, US
Aaron Rulison - Los Altos CA, US
Wesley B. Dong - Belmont CA, US
Andrew Fabans - Los Gatos CA, US
Allen Boronkay - San Jose CA, US
Edward Donlon - San Jose CA, US
Robert Moti - San Jose CA, US
Michael Slater - Modesto CA, US
Steven A. Sundberg - San Francisco CA, US
Michael R. Knapp - Palo Alto CA, US

Canon U.S. Life Sciences, Inc. - Rockville MD
CALIPER LIFE SCIENCES, INC. - MOUNTAIN VIEW CA

G01N 25/04
B01L 3/00
B01L 7/00
C12Q 1/6862
G01N 21/33
G01N 21/64
C12Q 1/6844
C12Q 1/686
C12Q 1/68

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

20150065360, Mar 5, 2015

Nov 11, 2014

14/538379

- San Diego CA, US
Jonathan Benton - Apex NC, US
Deborah Boles - Cary NC, US

ADVANCED LIQUID LOGIC, INC. - San Diego CA

C12N 15/10
C12Q 1/70
C12Q 1/68

506 5, 506 38

The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

20140093879, Apr 3, 2014

Mar 15, 2013

13/840994

- Rockville MD, US
Deborah John Boles - Cary NC, US
Aaron Rulison - Los Altos CA, US
Wesley B. Dong - Belmont CA, US
Andrew Fabans - Los Gatos CA, US
Allen Boronkay - San Jose CA, US
Edward Donlon - San Jose CA, US
Robert Moti - San Jose CA, US
Michael Slater - Modesto CA, US

Canon U.S. Life Sciences, Inc. - Rockville MD

C12Q 1/68

435 612, 4352872, 436 94

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.