Deane L Falcone

7547520, Jun 16, 2009

Dec 2, 2005

11/291995

Deane Louis Falcone - Lexington KY, US
John M. Littleton - Lexington KY, US

University of Kentucky Research Foundation - Lexington KY

G01N 33/60

435 71

This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to create a means of product discovery based on the biosynthetic capacity of plants. The materials to create an activation mutagenesis include incorporation of enhancer sequences from a plant viral promoter at random places in the plant genome via mediated DNA transfer (T-DNA). The usefulness is that genes in the immediate vicinity of the incorporation were activated which allows for immediate screening of the mutagenized plant cells. Additionally, the usefulness includes relevant areas of the genome were flanked by the inserted T-DNA which allows recovery of this area by standard molecular biology techniques. The method includes a procedure for screening large numbers of mutagenized plant cell cultures for activation of a relevant gene on the basis of the desired protein product on the basis of radioligand binding displacement assay.

7737327, Jun 15, 2010

Mar 9, 2004

10/795455

Deane Louis Falcone - Lexington KY, US
John M. Littleton - Lexington KY, US

The University of Kentucky Research Foundation - Lexington KY

C12N 15/82
C12N 15/84
C12N 15/10
C12N 15/12
C12N 15/38
C12N 15/62

800288, 800282, 800294, 800306, 8003173, 435 6, 435 71, 435 8, 435 697, 435421, 4354301, 435468, 435469

Methods for screening mutations that affect the synthesis of plant small molecules or compounds capable of activating a mammalian nuclear receptor protein and systems for rapidly assigning functionality to genes that regulate plant secondary metabolism are provided.

20100319094, Dec 16, 2010

Jun 12, 2009

12/483481

INDU BHUSHAN MAITI - LEXINGTON KY, US
DEANE L. FALCONE - WESTFORD MA, US
SUMITA RAHA - CHICAGO IL, US
H. MAELOR DAVIES - LEXINGTON KY, US

A01H 5/00
C12N 15/82
C12N 15/00
C12N 5/04

8003173, 800298, 435468, 4353201, 435419

A method for plastid transformation of a plant cell includes providing a delivery construct for transferring a passenger construct into a plant cell plastid for expression of a foreign DNA of interest therein. The delivery construct includes a nucleotide sequence under the control of a suitable promoter, for encoding at least one fusion protein which is a chloroplast transit peptide and a DNA binding domain. The passenger construct includes a nucleotide sequence encoding at least one foreign protein of interest or fragment thereof to be expressed in the plant cell, under the control of a plastid-specific promoter.

6989236, Jan 24, 2006

Jan 15, 2002

10/045677

Deane Louis Falcone - Lexington KY, US
John M. Littleton - Lexington KY, US

University of Kentucky Research Foundation - Lexington KY

C12N 15/82
G01N 33/534

435 71, 435 6, 435468, 435469

This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to create a means of product discovery based on the biosynthetic capacity of plants. The materials to create an activation mutagenesis include incorporation of enhancer sequences from a plant viral promoter at random places in the plant genome via mediated DNA transfer (T-DNA). The usefulness is that genes in the immediate vicinity of the incorporation were activated which allows for immediate screening of the mutagenized plant cells. Additionally, the usefulness includes relevant areas of the genome were flanked by the inserted T-DNA which allows recovery of this area by standard molecular biology techniques. The method includes a procedure for screening large numbers of mutagenized plant cell cultures for activation of a relevant gene on the basis of the desired protein product on the basis of radioligand binding displacement assay.

20210378161, Dec 9, 2021

Jun 4, 2021

17/339675

- Millis MA, US
Deane L. Falcone - Medway MA, US

A01B 79/02
A01C 7/06
A01C 7/18
G06F 3/0482

Described herein is a farm management system and related apparatus and operations including providing environmental monitoring and control systems, tracking seed sources, managing cultivation, growth, and harvest, improving enclosure operations, and managing system data. Example farm management systems may include a physical growing unit, a capacity control circuit structured to interpret a growing capacity description, and a growth support circuit structured to determine a planting instruction value in response to the growing capacity description. The physical growing unit may be responsive to the planting instruction value to initiate a growing support operation.

20160040176, Feb 11, 2016

Aug 7, 2015

14/821461

- Lexington KY, US
Deane Falcone - Lexington KY, US
Dustin Brown - Lexington KY, US
Samir Gunjan - Lexington KY, US
Trent Rogers - Lexington KY, US

C12N 15/82

Certain embodiments of the invention provide a method comprising: