David H Canter

7314542, Jan 1, 2008

Sep 22, 2005

11/234054

Daniel D. Smolko - Jamul CA, US
Paul D. Swanson - Santee CA, US
Dalibor Hodko - Poway CA, US
David Canter - San Diego CA, US
Robert W. Haigis - San Diego CA, US
Tricia Patterson - San Diego CA, US

Nanogen, Inc. - San Diego CA

G01N 27/447

204450, 204409, 435 6

Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.

7582421, Sep 1, 2009

Sep 16, 2002

10/245206

Ronald G. Sosnowski - Coronado CA, US
Michael I. Nerenberg - La Jolla CA, US
David M. Canter - San Diego CA, US
Ray R. Radtkey - San Diego CA, US
Ling Wang - San Diego CA, US
James P. O'Connell - Solana Beach CA, US

Nanogen, Inc. - San Diego CA

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00

435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253, 536 2532

This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.

7601493, Oct 13, 2009

Jul 24, 2003

10/627950

Ray R. Radtkey - San Diego CA, US
Lance C. Held - San Marcos CA, US
Robert B. Wallace - Escondido CA, US
Karen Menge - San Diego CA, US
David Canter - San Diego CA, US

Nanogen, Inc. - San Diego CA

C12Q 1/68
C12P 19/34
C12M 1/34
C07H 21/02
C07H 21/04

435 6, 435 911, 435 912, 4352872, 536 231, 536 243, 536 2433

An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.

20010014449, Aug 16, 2001

Apr 12, 1999

09/291129

MICHAEL I. NERENBERG - LA JOLLA CA, US
DAVID M. CANTER - SAN DIEGO CA, US
RAY R. RADTKEY - SAN DIEGO CA, US
LING WANG - San Diego CA, US
JAMES O'CONNELL - Solana Beach CA, US

C12Q001/68

435/006000, 422/050000, 422/068100

Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.

20100167960, Jul 1, 2010

Oct 7, 2009

12/574875

Ray R. Radtkey - San Diego CA, US
Lance C. Held - San Marcos CA, US
Robert B. Wallace - Escondido CA, US
Karen Menge - San Diego CA, US
David Canter - San Diego CA, US

C40B 60/12

506 39

An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.