Inventors:
Dagmar Bartos - Portland OR
Donald D. Trunkey - Oregon City OR
Angelo A. Vlessis - Portland OR
Assignee:
The State of Oregon Acting by and Through the State Board of Higher
Education on Behalf of Oregon Health Sciences University - Portland OR
International Classification:
C12M 134
C12Q 116
Abstract:
A simple device functioning as a radiorespirometer is a petri dish with a modified cover for quantitating an amount of CO. sub. 2 evolved by living cultured cells. The device is a plastic culture dish having a culture surface circumscribed by a continuous sidewall. A removable flat plastic cover has a flat inside face and an annular collar around the face that fits against a sidewall. The collar is maintained in substantially gas-tight relationship against the sidewall by an elastic band or tightly mating surfaces. An opening through the cover is sealed by a gas-impervious material, such as a plastic film, that can be penetrated by a needle to introduce acid into the dish for terminating culture growth and lysing the cells. A collector tube is removably secured to the inside face of the cover and contains a CO. sub. 2 trapping material, such as hyamine (methylbenzethonium hydroxide).