US Patent:
20050158812, Jul 21, 2005
Inventors:
Elizabeth Gomez - Chanhassen MN, US
Curtis Shellum - Rochester MN, US
International Classification:
G01N033/543
US Classification:
435007930, 435007100, 436501000, 436518000
Abstract:
An immunoassay method and test kit for detecting autoantibody in a patient sample, such as autoantibodies that interferes with the complexation of intrinsic factor with vitamin B12. A labeled receptor, i.e., intrinsic factor, is mixed with the sample and then with an anti-intrinsic factor antibody that binds competitively with the autoantibody to intrinsic factor bound to a solid phase is added and presence of autoantibody in the sample is determined.