US Patent:
20080167261, Jul 10, 2008
Inventors:
Christopher R. Sclimenti - La Jolla CA, US
International Classification:
A61K 31/711
C12N 9/24
C07H 21/04
C12N 15/87
US Classification:
514 44, 435200, 536 232, 435462
Abstract:
The present invention describes methods of identifying altered recombinases and compositions thereof, wherein at least one amino acid is different from a parent, wild-type recombinase and the altered recombinase has improved recombination efficiency towards wild-type and/or pseudo att site sequences relative to the parent, wild-type recombinase. The present invention also includes methods of modifying the genomes of cells using the altered recombinases, including methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell.