Inventors:
Charles E. Verostko - Houston TX
James E. Atwater - Eugene OR
James R. Akse - Roseburg OR
Jeffrey L. DeHart - Myrtle Creek OR
Richard R. Wheeler - Myrtle Creek OR
Assignee:
The United States of America as represented by the Administrator of the
National Aeronautics and Space Administration - Washington DC
International Classification:
C12Q 154
C12Q 128
C12Q 126
G01N 2176
Abstract:
A "reagentless" chemiluminescent biosensor and method for the determination of hydrogen peroxide, ethanol and D-glucose in water. An aqueous stream is basified by passing it through a solid phase base bed. Luminol is then dissolved in the basified effluent at a controlled rate. Oxidation of the luminol is catalyzed by the target chemical to produce emitted light. The intensity of the emitted light is detected as a measure of the target chemical concentration in the aqueous stream. The emitted light can be transmitted by a fiber optic bundle to a remote location from the aqueous stream for a remote reading of the target chemical concentration.
