Charles A Lissandrello

20210220823, Jul 22, 2021

Oct 12, 2018

16/755505

- Cambridge MA, US
Charles A. Lissandrello - Newtonville MA, US
Andrew P. Magyar - Arlington MA, US
Erin Rosenberger - Quincy MA, US

B01L 3/00
C12Q 1/6874
B01L 7/00
C12Q 1/6837
B04B 5/04
B04B 3/00

Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.

20200316601, Oct 8, 2020

Apr 3, 2020

16/839365

- Cambridge MA, US
Jason Fiering - Cambridge MA, US
Rebecca Christianson - Cambridge MA, US
Jason Durant - Cambridge MA, US
Charles Lissandrello - Cambridge MA, US

B01L 3/00

A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

20200071727, Mar 5, 2020

Aug 30, 2019

16/557820

- Cambridge MA, US
Charles A. Lissandrello - Newtonville MA, US
Jenna L. Balestrini - Boston MA, US
Jonathan R. Coppeta - Windham NH, US
Patricia A. Swierk - Cambridge MA, US

C12N 15/87
C12Q 1/02
C12M 1/42
B01L 3/00

Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.

20190292565, Sep 26, 2019

Mar 20, 2019

16/359626

- Cambridge MA, US
Charles A. Lissandrello - Newtonville MA, US
Jenna Leigh Balestrini - Boston MA, US
Ryan A. Dubay - Ludlow MA, US

C12N 15/87
C12N 13/00
C12N 15/85
C12M 1/42

A system for sequential exposure of particles to different fluid streams includes an acoustic actuator device for acoustically driving one or more substrates and a microchannel device of the one or more substrates that receive particles in a first flowing fluid, moves the particles to a second flowing fluid, then moves the particles out of the second flowing fluid using acoustic radiation generated by the acoustic actuator device. The system can control residence times in the streams. According to one use, the first flowing fluid is a cell buffer and the second flowing media is an electroporation buffer. An electroporation system is placed in or downstream of the acoustic actuator device. However, in other uses, the second flowing media might be a wash buffer.

20180133506, May 17, 2018

Nov 13, 2017

15/811429

- Cambridge MA, US
Joseph J. Register - Cambridge MA, US
Parshant Kumar - Cambridge MA, US
Carlos A. Segura - Cambridge MA, US
Charles A. Lissandrello - Cambridge MA, US
John J. LeBlanc - Cambridge MA, US

A61N 5/06
A61B 5/00

This disclosure provides a device that can include a first compliant optrode. The first compliant optrode can include a stack of flexible waveguide materials providing a first optical interface and configured to be introduced into a tissue sample. The stack of flexible waveguide materials can have a thickness of less than about 100 microns. The first compliant optrode can be substantially linear and can be configured to bend at a turn radius of less than about 300 microns.

20180136389, May 17, 2018

Nov 13, 2017

15/811203

- Cambridge MA, US
Joseph J. Register - Cambridge MA, US
Parshant Kumar - Cambridge MA, US
Carlos A. Segura - Cambridge MA, US
Charles A. Lissandrello - Cambridge MA, US
John J. LeBlanc - Cambridge MA, US

G02B 6/02
G02B 1/04
G02B 6/26
B05D 1/00
B05D 3/10
B05D 3/12
A61N 5/06

The material stack of the present disclosure can be used for fabricating optical waveguides that are thin and flexible, and that can bend light around small turns. The stack of materials can include a polymer core and a cladding, which together can create a large difference in refractive index. As a result, light can remain within the core even when bent around radii where standard glass fibers could fail.