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Charles E Hodgman

from Acton, MA
Age ~39

Charles Hodgman Phones & Addresses

  • Acton, MA
  • Evanston, IL
  • 7 Flanagan Dr, Framingham, MA 01701 (508) 877-6434
  • Natick, MA
  • West Lafayette, IN
  • Kalamazoo, MI

Industries

Construction

Resumes

Resumes

Charles Hodgman Photo 1

Owner At C.s. Hodgman & Sons/ Carpentry

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Location:
Greater Boston Area
Industry:
Construction

Publications

Us Patents

Substrate Replenishment And Byproduct Removal Improve Yeast Cell-Free Protein Synthesis

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US Patent:
20150259757, Sep 17, 2015
Filed:
Mar 16, 2015
Appl. No.:
14/659206
Inventors:
- Evanston IL, US
Jennifer A. Schoborg - Evanston IL, US
Charles Eric Hodgman - Evanston IL, US
Mark J. Anderson - Wilmette IL, US
International Classification:
C12Q 3/00
C12P 21/00
Abstract:
Methods and kits are provided for calibrating a cell-free protein synthesis reaction for optimal activity. The method includes the steps of providing an extract competent for cell-free protein synthesis (CFPS); performing cell-free protein synthesis with the extract; measuring a first reaction end-point where in vitro protein synthesis plateaus; measuring a second reaction end-point where Energy Charge of the extract declines to a level in a range from about 0.40 to about 0.80 of Energy Charge of a control extract; and adjusting Energy Charge of the extract to a level in a range from about 0.80 to about 1.0 of the control extract.

Methods For Cell-Free Protein Synthesis

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US Patent:
20140295492, Oct 2, 2014
Filed:
Mar 14, 2014
Appl. No.:
14/213390
Inventors:
- Evanston IL, US
CHARLES E. HODGMAN - Evanston IL, US
RUI GAN - Evanston IL, US
Assignee:
Northwestern University - Evanston IL
International Classification:
C12P 21/00
C12N 9/12
US Classification:
435 681, 435194
Abstract:
Cell-free protein synthesis systems and methods of using the same for producing in vitro protein materials in high yield are disclosed. The cell-free protein synthesis platform includes (a) a cellular extract prepared from mid-exponential to late-exponential batch cultures in the range from about 6 ODto about 18 ODor fed-batch cultures harvested in mid-exponential to late-exponential phase; (b) a reaction buffer; and (c) a translation template or (c′) a transcription template from which a translation template can be prepared in situ with an RNA polymerase. A method of performing high-throughput protein synthesis in vitro is also provided that utilizes a combined transcription/translation reaction with the cell-free protein synthesis platform from , an RNA polymerase and a transcription template prepared from a source DNA using an amplification procedure.
Charles E Hodgman from Acton, MA, age ~39 Get Report