US Patent:
20210214673, Jul 15, 2021
Inventors:
- Livermore CA, US
Kaiwan Chear - Livermore CA, US
Bruce Leisz - San Jose CA, US
David Eberhart - Santa Clara CA, US
John Bashkin - Fremont CA, US
International Classification:
C12M 1/33
C12M 1/00
C12M 1/34
G01N 1/40
G01N 1/31
G01N 1/28
Abstract:
A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.