Inventors:
Bruce W. Calnek - Ithaca NY
Benjamin Lucio-Martinez - Ithaca NY
Carol Cardona - Davis CA
Raymond W. Harris - Dryden NY
Karel A. Schat - Ithaca NY
Assignee:
Cornell Research Foundation, Inc. - Ithaca NY
International Classification:
C12N 1500
US Classification:
435325, 435 5, 4352351, 435236, 435239
Abstract:
The present invention relates to a method of propagating chicken infectious anemia virus (CIAV). This method involves providing a Mareks disease chicken cell lineâCU147 culture and inoculating the culture with a chicken infectious anemia virus under conditions effective to propagate the virus in the culture. The present invention also relates to methods of isolating, identifying, and quantifying chicken infectious anemia virus in a sample which includes providing a biological sample, providing a Mareks disease chicken cell lineâCU147 culture, incubating the culture with the biological sample under conditions effective to allow a chicken infectious anemia virus to infect the culture, and isolating, identifying, or quantifying the virus in the culture. The present invention also relates to a high titer vaccine formulation for chicken infectious anemia virus which includes an immunologically effective amount of chicken infectious anemia virus propagated in a Mareks disease chicken cell lineâCU147 culture. Another aspect of the present invention is a method of immunizing poultry against chicken infectious anemia virus which includes administering a vaccine prepared from chicken infectious anemia virus propagated in a Mareks disease chicken cell lineâCU147 culture in an amount effective to induce an immune response to the virus.