Brett T Bowman

20150211079, Jul 30, 2015

Jul 11, 2013

14/414688

- San Diego CA, US
Brett Bowman - San Diego CA, US
Xianqun Wang - San Marcos CA, US
Mingjie Wang - Bloomington IN, US

C12Q 1/70
G06F 19/18

Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.