Inventors:
Robert H. Reeves - Tallahassee FL
Brenda W. Bennison - Tallahassee FL
Paul A. LaRock - Baton Rouge LA
Assignee:
Florida State University - Tallahassee FL
International Classification:
C12Q 168
C07H 2104
Abstract:
The present invention relates to species-specific DNA probes specific for Vibrio vulnificus and Vibrio cholerae. The DNA probes of the present invention specifically detects Vibrio vulnificus or Vibrio cholerae in a mixed bacterial sample based on unique ribosomal RNA nucleotide sequences. When the DNA probes of the present invention are tagged with a labeled molecule such as a fluorescent label, it affords direct and immediate visualization of individual bacterial cells, and a rapid method of detection of bacterial infection in humans and shellfish without culturing.