US Patent:
20110059476, Mar 10, 2011
Inventors:
Hye-Won Shin - Irvine CA, US
Brandon J. Umber - Corona Del Mar CA, US
Donald R. Blake - Irvine CA, US
Dan M. Cooper - Irvine CA, US
International Classification:
C12Q 1/02
C12M 1/34
Abstract:
A bioreactor for sampling the headspace above cells includes a glass reactor vessel having an interior space and a plurality of valves operatively coupled to the interior space of the glass reactor vessel. The bioreactor includes a sample holder configured to reside in the interior space. The interior space includes a charge of air with a known composition of volatile organic gases and, in some instances, doped with carbon dioxide. The sample holder may hold cells, bacteria, or viruses. The headspace may be sampled after incubation to determine the VOC content or content of specific VOC constituents. Examples include acetaldehyde and hexanaldehyde.