Boris Y Sabirzhanov

Baltimore, MD

Research

University of Maryland Baltimore
Research Assistant Professor
The University of South Dakota Oct 2004 - Oct 2010
Postdoctoral Fellow

Molecular and Cellular Biology
In Vitro Screening of Genes and Compounds
Cell Culture
Planning and Performing of Experiments
Upstream Process Development
Assays and Methods Development
Training Colleagues In Molecular Biology Methods
Working on Collaborative Projects
Project Design and Management
Microsoft Office
Dna
Gel Electrophoresis
Southern Blotting
Preparation of Radiolabeled Dna Probes
Genome Walking
Dna Sequencing
Rna Isolation
Northern Blot
Cdna Synthesis
Rnai
Design and Validation of Sirna
Microrna
Pcr
Design and Validation of Primers and Real Time Pcr Assays
Rapid Amplification of Cdna Ends
Real Time Pcr
Constructing of Recombinant Dna Molecules
Preparation of Primary Neuronal and Microglia Cultures
Sds Page
Western Blotting
Production of Recombinant Proteins
Protein Purification
Immunoprecipitation
Transfection
Cell Death and Viability Assays
Immunostaining
Microscopy
Production of Viral Particles
Chromatin Immunoprecipitation
Dna Methylation Analysis
Bioinformatics
Statistics
Assay Development
In Vitro
Protein Chemistry

Russian
English

School of Medicine

Oct 2010 to 2000
Postdoctoral fellow
School of Medicine

Oct 2004 to Oct 2010
Postdoctoral fellow
R&D Department

1999 to 1999
Intern

Institute of Biochemistry and Genetics
1999 to 2003
Ph.D. in Molecular Biology
Bashkir State University
1994 to 1999
Master's in Biochemistry

DNA: DNA purification, DNA gel electrophoresis, restriction analysis of DNA, Southern blotting, DNA sequencing, preparation of radiolabeled DNA probes, single-strand conformation polymorphism analysis; genome walking RNA: RNA isolation, quantification and quality control of RNA, cDNA synthesis, Northern blot RNAi: Design and validation of siRNA; construction of siRNA rescue plasmid microRNA: isolation, profiling of miRs expression, functional analysis, mRNA targets prediction PCR: PCR, RT-PCR, rapid amplification of cDNA ends, real-time PCR; design and validation of primers and real-time PCR assays; PCR diagnostic of infections Constructing of recombinant DNA molecules: Cloning of PCR products and DNA fragments into plasmid vectors; Construction of plasmid vectors for gene expression into E. coli, yeast and eukaryotic cell lines; Site directed mutagenesis Proteins: SDS PAGE, Western blotting; Production of recombinant proteins in E. coli, yeast and eukaryotic cell lines, cell free in vitro protein expression; Protein purification, affinity chromatography, tandem affinity purification, immunoprecipitation; Enzyme activity analysis Cell culture: preparation of primary neuronal and microglia cultures, manipulation with variety of cell lines, including neuronal cell lines; transfection of cell cultures with plasmids, stable cell line generation, siRNA and microRNA mimics; in vitro cell-based bioassays: Lactate Dehydrogenase, calcein, MTT, caspase-3 activity and Propidium Iodide uptake; immunostaining; microscopy Upstream process development: Cloning of target gene; construction of vectors for gene expression into E. coli, yeast and eukaryotic cell lines; Site directed mutagenesis and construction of fused genes; Clone and strain selection; Expression optimization; Development of strains with stable expression levels Production of viral particles: adeno-associated virus (AAV); lentiviruses; bacteriophage lambda and bacteriophage M13 Epigenetics: Chromatin immunoprecipitation (ChIP); DNA methylation analysis by bisulfite genomic sequencing and qPCR In vivo: Mice handling; genotyping; transcardial perfusion; brain dissection Bioinformatics: Analysis of nucleotide and amino acid sequences, sequence alignment and assembly; Design PCR primers and probes for sequenced and non-sequenced species; Analysis of microarray data; Function, pathway and network analysis of complex data by Ingenuity Systems (IPA) application Computer skills: Microsoft Office; Photoshop; sequence analysis: Lasergene (DNASTAR) and online tools such as NCBI, EMBL; pathway analysis: Ingenuity Pathway Analysis (IPA); gene expression analysis: GeneSpring GX; statistical analysis: GraphPad Prism, SigmaStat.