Search

Barton Nancy E Holmquist

from Eagle, NE
Age ~81
Get Report
Also known as:
Barton Nancy Holmquist, Bart Holmquist, Bart Holmquest, Bart Holmauist, Bart T, Timothy Prusha
Phone and address:
1333 Prairie View Rd, Eagle, NE 68347
(402) 780-5126
Related to:
Brett Holmquist, 53
Connected to:
Angela Holmquist, 52Angelic D Holmquist, 53Brad L Holmquist, 60Brian M Holmquist, 54Bruce L Holmquist, 71Carey R Holmquist, 69Carl E Holmquist, 43Charles W Holmquist, 69Chris Holmquist, 53Clifton N Holmquist, 88

Barton Holmquist Phones & Addresses

  • 1333 Prairie View Rd, Eagle, NE 68347 (402) 780-5126
  • Canton, MA
  • 3820 46Th St, Lincoln, NE 68524
  • Fairbury, NE
  • Waltham, MA
  • Burlington, MA

Publications

Us Patents

Clostripain Catalyzed Amidation Of Peptides

View page
US Patent:
6461834, Oct 8, 2002
Filed:
Dec 16, 1998
Appl. No.:
09/212663
Inventors:
Dan Dormady - Omaha NE
Jay S. Stout - Lincoln NE
Daniel J. Strydom - Lincoln NE
Barton Holmquist - Lincoln NE
Fred W. Wagner - Walton NE
Assignee:
Bionebraska, Inc. - Lincoln NE
International Classification:
C12P 2106
US Classification:
435 681, 435220
Abstract:
The invention provides a method of producing a polypeptide having a C-terminal -carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an -carboxamide group (C-terminal âArg-NH â). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.

Method For Universal Enzymatic Production Of Bioactive Peptides

View page
US Patent:
7335486, Feb 26, 2008
Filed:
Nov 24, 2004
Appl. No.:
10/997074
Inventors:
Fred W. Wagner - Walton NE, US
Peng Luan - Omaha NE, US
Yuannan Xia - Lincoln NE, US
Daniel Strydom - Lincoln NE, US
Edwin H. Merrifield - Lynnwood WA, US
Mary J. Bossard - Madison AL, US
Barton Holmquist - Eagle NE, US
Jin Seog Seo - Brampton, CA
Assignee:
Restoragen, Inc. - Lincoln NE
International Classification:
C12P 21/06
US Classification:
435 681, 435220
Abstract:
The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

Peptide Amidation Process

View page
US Patent:
7462690, Dec 9, 2008
Filed:
Nov 24, 2004
Appl. No.:
10/997081
Inventors:
Barton Holmquist - Eagle NE, US
Daniel Strydom - Lincoln NE, US
Assignee:
Restoragen, Inc. - Lincoln NE
International Classification:
C07K 1/107
US Classification:
530345
Abstract:
The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.

Method For Universal Enzymatic Production Of Bioactive Peptides

View page
US Patent:
7498148, Mar 3, 2009
Filed:
Nov 21, 2007
Appl. No.:
11/944165
Inventors:
Fred W. Wagner - Walton NE, US
Peng Luan - Fishers IN, US
Yuannan Xia - Lincoln NE, US
Daniel Strydom - Lincoln NE, US
Edwin H. Merrifield - Lynnwood WA, US
Mary J. Bossard - Madison AL, US
Barton Holmquist - Eagle NE, US
Jin Seog Seo - Brampton, CA
Assignee:
Restoragen, Inc. - Lincoln NE
International Classification:
C12P 21/06
US Classification:
435 681, 435220
Abstract:
The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

Polypeptide Cleavage Process

View page
US Patent:
7592432, Sep 22, 2009
Filed:
Nov 24, 2004
Appl. No.:
10/997822
Inventors:
Barton Holmquist - Eagle NE, US
Daniel Strydom - Lincoln NE, US
Jin Seog Seo - Brampton, CA
Kirk Cryer - Madison WI, US
Assignee:
Restoragen, Inc. - Lincoln NE
International Classification:
C08H 1/00
C12N 1/20
C12N 15/63
US Classification:
530402, 435 691, 4352521, 530333
Abstract:
The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is between about 1 to about 22 molar.

Polypeptide Cleavage Process

View page
US Patent:
7771966, Aug 10, 2010
Filed:
Nov 24, 2004
Appl. No.:
10/997762
Inventors:
Jin Seog Seo - Mississauga, CA
Daniel Strydom - Lincoln NE, US
Barton Holmquist - Eagle NE, US
Assignee:
Medtronic, Inc. - Minneapolis MN
International Classification:
C12P 21/06
US Classification:
435 691, 435 6, 4353201, 435 71
Abstract:
A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent.

Methods And Constructs For High Yield Expression Of Clostripain

View page
US Patent:
8003362, Aug 23, 2011
Filed:
Nov 24, 2004
Appl. No.:
10/997697
Inventors:
Fred W. Wagner - Walton NE, US
Peng Luan - Fishers IN, US
Yuannan Xia - Lincoln NE, US
Barton Holmquist - Eagle NE, US
Assignee:
Medtronic Inc. - Minneapolis MN
International Classification:
C12N 9/48
C12N 1/20
C12N 15/74
C07K 1/00
US Classification:
435212, 43525233, 435488, 530350
Abstract:
The invention provides methods and nucleic acid constructs to express clostripain. The source of the coding region for recombinantly expressed clostripain is.

Composition For Palladium-Mediated Cleavage Of Peptides Containing Cxc, Cxh Or Chm Sequences

View page
US Patent:
8153762, Apr 10, 2012
Filed:
Aug 28, 2008
Appl. No.:
12/199902
Inventors:
Barton Holmquist - Eagle NE, US
Daniel J. Strydom - Lincoln NE, US
Jin Seog Seo - Mississauga, CA
Assignee:
Medtronic, Inc. - Minneapolis MN
International Classification:
C07K 1/107
US Classification:
530345
Abstract:
The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X-cysteine sequence, wherein Xis the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X-X, wherein Xis any amino acid, Xis an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X-cysteine sequence.
Control profile
Barton Nancy E Holmquist from Eagle, NE, age ~81 Get Report