Alicia Lacy Carlson

20110044910, Feb 24, 2011

Mar 27, 2009

12/867141

Charles P. Lin - Arlington MA, US
Alicia L. Carlson - Somerville MA, US
Clemens Alt - Watertown MA, US
David P. Biss - Brighton MA, US
Costas M. Pitsillides - Boston MA, US
Chunqiang Li - Belmont MA, US

THE GENERAL HOSPITAL CORPORATION D/B/A MASSACHUSEETS GENERAL HOSPITAL - BOSTON MA

A61K 49/00
G01J 1/58

424 96, 2504581, 250200

The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest.

20140031647, Jan 30, 2014

Sep 30, 2013

14/041047

Alicia L. Carlson - Medford MA, US
Clemens Alt - Watertown MA, US
David P. Biss - Brighton MA, US
Costas M. Pitsillides - Larnaca, CY
Li Chunqiang - Belmont MA, US

The General Hospital Corporation D/B/A Massachusetts General Hospital - Boston MA

A61B 5/00

600317

The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest.