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Alexander V Kukhtin

from Middletown, MD
Age ~53
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Also known as:
Alexander V Kukhtina, Nataliya Kukhtina, Kukhtin Alexander, Kukhtina Nataliya, Alexander H
Phone and address:
16 Bankbarn Cir, Middletown, MD 21769
Related to:
Nataliya N Kukhtina, 53
Connected to:
Sergey V Kukhtin, 62Myrna E Kuki, 72Dilber Kukic, 49Francis C Kukic, 80Jasmin Kukic, 52Mustafa Kukic, 65Zeljko Kukic, 54Peter M Kukich, 67Richard J Kukiela, 40Dale J Kukielka, 61

Alexander Kukhtin Phones & Addresses

  • 16 Bankbarn Cir, Middletown, MD 21769
  • 4915 Meridian Way #12, Frederick, MD 21703
  • 17648 Gilbert Dr, Lockport, IL 60441
  • Oceanside, CA
  • Hinsdale, IL
  • Willeys, IL
  • Downers Grove, IL
  • Willowbrook, IL
  • 4915A Meridian Way APT 12, Frederick, MD 21703

Work

Company: Akonni biosystems Jan 2005 Position: Senior scientist

Education

Degree: Doctorates, Doctor of Philosophy School / High School: Lomonosov Moscow State University (Msu) 1988 to 1993 Specialities: Philosophy, Chemistry

Skills

Molecular Biology • Assay Development • Genomics • Lifesciences • Chemistry • Microarray Analysis • Proteomics • Biotechnology • Biochemistry • Dna Microarray • Multiplex Pcr • Pcr Primer Design • Dna • Enzyme Kinetics • Pcr • Protein Purification • Enzymes • Immunoassays • Cell Culture • Molecular Diagnostics • Genetics • High Throughput Screening • Dna Sequencing • Dna Extraction • Life Sciences • Polymerase Chain Reaction

Languages

Russian • English

Emails

a***[email protected]

Industries

Biotechnology

Resumes

Resumes

Alexander Kukhtin Photo 1

Senior Scientist

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Location:
400 Sagner Ave, Frederick, MD 21701
Industry:
Biotechnology
Work:
Akonni Biosystems
Senior Scientist
Argonne National Laboratory Jan 2000 - Jan 2005
Researcher
Engelhardt Institute of Molecular Biology Apr 1996 - Jan 2000
Researcher
Education:
Lomonosov Moscow State University (Msu) 1988 - 1993
Doctorates, Doctor of Philosophy, Philosophy, Chemistry
Skills:
Molecular Biology
Assay Development
Genomics
Lifesciences
Chemistry
Microarray Analysis
Proteomics
Biotechnology
Biochemistry
Dna Microarray
Multiplex Pcr
Pcr Primer Design
Dna
Enzyme Kinetics
Pcr
Protein Purification
Enzymes
Immunoassays
Cell Culture
Molecular Diagnostics
Genetics
High Throughput Screening
Dna Sequencing
Dna Extraction
Life Sciences
Polymerase Chain Reaction
Languages:
Russian
English

Publications

Us Patents

Biochip Reader With Enhanced Illumination And Bioarray Positioning Apparatus

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US Patent:
7288227, Oct 30, 2007
Filed:
Jul 14, 2003
Appl. No.:
10/619284
Inventors:
Gennadiy Yershov - Hinsdale IL, US
Oleg Alferov - Woodridge IL, US
Alexander Kukhtin - Hinsdale IL, US
Assignee:
UChicago Argonne LLC - Chicago IL
International Classification:
G01N 21/64
US Classification:
422 8211, 2504581, 2504611, 422 8205, 422 8206, 422 8208, 422 8209, 422104, 436172
Abstract:
A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage.

Nucleic Acid Sample Purification And Enrichment With A Thermo-Affinity Microfluidic Sub-Circuit

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US Patent:
7985716, Jul 26, 2011
Filed:
May 3, 2007
Appl. No.:
11/744112
Inventors:
Gennadiy M. Yershov - Willowbrook IL, US
Alexander Kukhtin - Lockport IL, US
Boris K. Chernov - Burr Ridge IL, US
Julia B. Golova - Willowbrook IL, US
Darrell P. Chandler - Manchester MI, US
Assignee:
UChicago Argonne, LLC - Chicago IL
International Classification:
C40B 60/10
US Classification:
506 38, 506 3, 435 6
Abstract:
Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.

Method For Fabrication Of Biochips With A Macroporous Polymer Substrate

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US Patent:
20050042363, Feb 24, 2005
Filed:
Aug 18, 2003
Appl. No.:
10/642768
Inventors:
Alexander Kukhtin - Lockport IL, US
Boris Chernov - Willowbrook IL, US
Julia Golova - Willowbrook IL, US
Gennadiy Yershov - Willowbrook IL, US
Margaret Gemmell - Western Springs IL, US
International Classification:
B05D003/00
A61L002/00
US Classification:
427008000, 427002100
Abstract:
Macroporous polymer substrates have greater immobilization capacity for large biomolecules and better access for analytes than substrates used previously for microarrays. Microarrays are fabricated with macroporous polymer substrates, and nucleic acids, proteins and peptides are immobilized in the substrate. Microarrays with macroporous polymer substrates are useful in immunoassays, drug discovery studies and other biotechnological applications that involve large scale macromolecular interactions. Nucleic acid hybridizations, protein binding, and antigen-antibody interactions are analyzed using microarrays with macroporous polymer substrates.

Methods And Compositions For Synthesis Of 3'-Aminolinkers

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US Patent:
20050208199, Sep 22, 2005
Filed:
Mar 18, 2004
Appl. No.:
10/803280
Inventors:
Julia Golova - Willowbrook IL, US
Boris Chernov - Willowbrook IL, US
Alexander Kukhtin - Lockport IL, US
International Classification:
C12M001/34
C07H021/04
US Classification:
427002110, 536023100, 435287200
Abstract:
Methods and compositions for 3′-aminomodifier CPG and 3′-aminomodified oligonucleotides are disclosed. Microarrays or biochips with 3′-aminomodified oligonucleotides are disclosed. A variety of detectable labels can be attached to 3′-aminomodified oligonucleotides.

Omega-Amino-Peg-Phosphoramidites And Conjugates Thereof

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US Patent:
20060063147, Mar 23, 2006
Filed:
Sep 21, 2004
Appl. No.:
10/945834
Inventors:
Boris Chernov - Willowbrook IL, US
Alexander Kukhtin - Lockport IL, US
Julia Golova - Willowbrook IL, US
International Classification:
C40B 40/08
US Classification:
435005000, 435006000, 536025300
Abstract:
ω-Amino-PEG conjugates, and processes and reagents for preparing ω-amino-PEG conjugates are described.

Covalent Attachment Of Biomolecules To Solid Supports By A Polymerization Method

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US Patent:
20070238163, Oct 11, 2007
Filed:
Apr 5, 2006
Appl. No.:
11/399034
Inventors:
Julia Golova - Burr Ridge IL, US
Boris Chernov - Burr Ridge IL, US
Alexander Kukhtin - Lockport IL, US
International Classification:
C12M 1/34
G01N 1/28
US Classification:
435287200, 427002110, 977902000
Abstract:
Described herein is a novel approach for the immobilization of one or more biomolecules or biomolecule complexes to solid supports. The method describes the modification of a solid support, if required, attaching functionality to a biomolecule or biomolecule complex, and linking the biomolecule or biomolecule complex to the solid support. The described complexes are rapidly produced while conserving both orientation and functional activity of the utilized biomolecule or biomolecule complex. Thus, materials produced by this method thus have applications as nanomaterials for biosensor fabrication, screening microarrays, high-throughput drug discovery, and as a tool for combinatorial chemistry, to name only a few.

Biochip Reader With Enhanced Illumination And Bioarray Positioning Apparatus

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US Patent:
6620623, Sep 16, 2003
Filed:
May 6, 2002
Appl. No.:
10/139842
Inventors:
Gennadiy Yershov - Hinsdale IL
Oleg Alferov - Woodridge IL
Alexander Kukhtin - Hinsdale IL
Assignee:
The University of Chicago - Chicago IL
International Classification:
G01N 2164
US Classification:
436172, 422 8208
Abstract:
A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage.
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Alexander V Kukhtin from Middletown, MD, age ~53 Get Report