US Patent:
20200032244, Jan 30, 2020
Inventors:
- Pleasanton CA, US
- Wilmington MA, US
Alexander Lovejoy - Newark CA, US
Bronwen Miller - Cape Town, ZA
Jo-Anne Elizabeth Penkler - Cape Town, ZA
Joseph Platzer - Oakland CA, US
Martin Ranik - Santa Clara CA, US
International Classification:
C12N 15/10
C12Q 1/6813
C12Q 1/6876
Abstract:
The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.